A pipette is a type of chemical dispenser used in the laboratory to measure and transport certain chemicals. There are different designs of pipettes, but they all work the same. The pipette is placed in a container filled with liquid, where it creates a vacuum. The vacuum suction sucks up a certain amount of liquid and distributes it into another. The pipettes are made with borosilicate, alumina silicate, or quartz.
Micropipettes are designed to measure and distribute small amounts of liquid. The micropipettes deliver between 1 and 1,000 microliters. They are often used in microinjection and measurement of experiments, as they are highly accurate.
A volumetric pipette, such as a volumetric flask, is used to measure and distribute a specific amount of liquid to add to another. A number of pipette sizes are accessible for measuring a fixed amount of liquid, with popular sizes of 10, 25, and 50 millilitres using the pipette. Volumetric pipettes are frequently used in titration experiments.
It is marked with a series of graduated lines to measure the amount of liquid before and after dispensation. It’s not as accurate as a whole pipette, but it serves its purpose.
The accuracy of the pipette depends on several factors. An inexperienced chemist can make mistakes that affect the measurements. Another factor is the type of liquid being pipetted. Regular use of corrosive liquids can damage the pipette pistons and O-rings, causing inaccurate measurements.
The pipette calibration service is an operation of a quality system. Pipettes are volumetric measuring instruments. The pipettes calibration reveals that they have low reproducibility and are very vulnerable to the method and technique’s errors. It is an instrument widely used in physicochemical, clinical, and R&D laboratories. The pipettes dose a specific volume using an injection mechanism, which can be manual or automatic.
Pipettes can be single-channel or multichannel, depending on the pipette’s number of fluid outlets. Special care must be taken to operate the piston of an automatic pipette smoothly and regularly. Pipetting is a delicate operation that requires proper and practical training to achieve consistent and accurate results. Even more so in matters of calibration since the object of the measurements is to determine the pipette’s error.
The pipette calibration service is provided includes the following:
- Automatic pipette calibration.
- Calibration of manual pipettes.
- Laboratory pipette calibration.
- Calibration of mechanical pipettes.
- Gilson pipette calibration.
- Brand pipette calibration.
- Calibration of Eppendorf pipettes.
Socorex pipette calibration.
In the calibration of the pipettes, duly certified standards of the mechanical area are used. The result of the pipette calibration is included in a calibration certificate, which must contain a series of minimum information and meet a series of conditions to be accepted and recognized.
Although they are not the only ones, for information purposes, three factors to take into account when calibrating the pipettes are:
Control over piston action: Operating the piston too fast can introduce air bubbles, causing an error in the amount of liquid dispensed.
The pipette is calibrated in the vertical position when the liquid is aspirated.
The pipette calibration certificates issued in the laboratory include all the information necessary to ensure the traceability of the measurements. The calculation of the measurement uncertainty is carried out according to the European guide.
What do you need?
- Pipettes that need to be calibrated.
- Distilled water.
- Clean glasses.
- Density table of distilled water with temperature.
- Fast precision or an analytical balance with a readability of at least 0.001g.
- Something to write down measurements (Book, Computer, etc.).
Calculator or software to calculate results
We recommend using an anti-vibration table. Or, at a minimum, make sure you are calibrating in a well-conditioned room with temperature control and good conditions (no vibration, temperature or atmospheric changes, static electricity, etc.).
The person doing the calibration should be accustomed to pipetting. For classes, allow students to practice proper pipetting techniques before calibrating the instrument. In either case, make sure your equipment is clean and functioning. Also, calibrate the balance in advance to make sure it is stable before starting measurement.
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